Effective nuclear reprogramming in mammals using CDK2 inhibitors
US6906238B2 · kind B2 · utility
0Cited by
13References
40Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Mar 15, 2001 |
| Grant date | Jun 14, 2005 |
| Priority date | — |
| Expiry date | Feb 15, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/873
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods of producing a cloned non-human mammalian nuclear transfer (NT) embryo and methods for producing a cloned non-human mammal. Embodiments of the methods include introducing donor genetic material into a metaphase I oocyte; introducing donor genetic material into a non-enucleated oocyte; introducing donor genetic material obtained from a donor cell that is at metaphase into an oocyte; introducing donor genetic material into an oocyte, and naturally activating the oocyte or the NT embryo; and introducing donor genetic material obtained from a donor cell that is at late G1 phase into an oocyte.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.