Protease for activating clotting factor VII

US6911334B2 · kind B2 · utility

Assignee

• ZLB Behring AG · CH

Inventors

• Jürgen Römisch · Marburg, DE
• Annette Feussner · Marburg, DE
• Hans-Arnold Stohr · Wetter, DE

Key dates

• Filing date: Dec 16, 2002
• Grant date: Jun 28, 2005
• Expiry date: Dec 16, 2022

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/96447
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A protease for activating the blood clotting factor VII is described, wherein the protease is inhibited by the presence of aprotinin and is increased in its activity by calcium ions and/or heparin or heparin-related substances, and wherein in SDS-PAGE, on subsequent staining in the non-reduced state, the protease has one or more brands in the molecular weight range from 50 to 75 kDa, and in the reduced state, the protease has a band at 40 to 55 kDa and one or more bands in the molecular weight range from 10 to 35 kDa. The proenzyme of this protease is also characterized. Further, a process for obtaining this protease and its use in hemorrhage prophylaxis or hemostasis is described. Moreover, a test system for the qualitative and quantitative detection of a protease which activates the blood clotting factor VII is described.