Targeted manipulation of genes in plants
US6911575B1 · kind B1 · utility
14Cited by
8References
20Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 26, 2000 |
| Grant date | Jun 28, 2005 |
| Priority date | — |
| Expiry date | May 26, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/53
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions for altering a nucleotide sequence of interest in a plant is provided. The method involves introducing a chimeric oligonucleotide into a plant cell, wherein the oligonucleotide comprises at least two intervening blocks of RNA residues with intervening DNA residues. The RNA blocks are homologous to a plant nucleotide sequence. The chimeric oligonucleotide is capable of folding to form a duplex oligonucleotide. The chimeric oligonucleotide is maintained within the nucleus of the plant whereby the alteration is introduced into the target sequences of the plant genome.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.