Methods of making L-amino acids in coryneform using the sigE gene

US6913908B2 · kind B2 · utility

Assignee

• Degussa AG · DE

Inventors

• Bettina Mockel · Bielefeld, DE
• Thoams Hermann · Bielefeld, DE
• Mike Farwick · Essen, DE
• Michael Binder · Steinhagen, DE
• Walter Pfefferle · Bodenwerder, DE

Key dates

• Filing date: Aug 24, 2001
• Grant date: Jul 5, 2005
• Expiry date: Apr 4, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12R2001/15
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates lo an isolated polynucleotide from Corynebacterium glutamicum comprising a polynucleotide sequence chosen from the group c insisting of (a) a polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO: 2; (b) a polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO: 2; (c) a polynucleotide which is complementary to the polynucleotides of(a) or (b), and (d) a polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of (a), (b), or (c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the sigE gene is present in enhanced form, and the use of polynucleotides which comprise the sequence according to the invention as hybridization probes.