Patent · US Expired

Isolation of DNA molecules

US6916919B2 · kind B2 · utility

0Cited by

4References

15Claims

0Family size

Assignee

Amersham Biosciences AB · SE

Inventors

Raf Lemmens · Uppsala, SE
Tomas Nyhammar · Uppsala, SE
Jan Berglof · Uppsala, SE
Joachim Stadler · Glückstadt, DE

Key dates

Filing date	Apr 15, 2002
Grant date	Jul 12, 2005
Priority date	—
Expiry date	Apr 15, 2022

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/101
WIPO fieldChemical engineering
WIPO sectorChemistry

Abstract

The present invention relates to a process for separating nucleic acid molecules, preferably open circular and supercoiled plasmid DNA and RNA molecules from each other, comprising the steps of providing a solution comprising the molecules; adsorbing the molecules to adsorbing groups on a carrier; and optionally washing the column with a suitable solution. The present process is especially suitable for large-scale isolation of supercoiled ccc DNA to be used in gene therapy.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.