Hybridization rate enhancement for substrate-bound specific nucleic acid-binding agents
US6919180B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 22, 2002 |
| Grant date | Jul 19, 2005 |
| Priority date | — |
| Expiry date | Jan 19, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B40/10
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The invention relates to kits and methods for hybridizing nucleic acids with a specific nucleic acid-binding agent, such as a complementary nucleic acid. Previously, others have hybridized a nucleic acid with such an agent bound to a substrate. The improved methods described herein comprise binding a polycationizable attractor compound to the substrate, in addition to the agent. Examples of suitable polycationizable attractor compounds include polypeptides, including those with tunable cationizable amino acid residues, such as histidine. Compositions, kits, devices, and methods that make use of this hybridization rate enhancement technology are disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.