Method for cloning and expression of Tth111II restriction endonuclease-methylase in E. coli
US6919194B2 · kind B2 · utility
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5Claims
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Key dates
| Filing date | Jan 8, 2003 |
| Grant date | Jul 19, 2005 |
| Priority date | — |
| Expiry date | Jan 7, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to recombinant DNA which encodes the Tth111II restriction endonuclease-methylase fusion protein (Tth111IIRM), expression of Tth111II restriction endonuclease-methylase fusion protein in E. coli cells containing the recombinant DNA, and purification of Tth111II endonuclease-methylase fusion protein to near homogeneity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.