Electrophoresis apparatus and method

US6923896B2 · kind B2 · utility

Assignees

• THE TEXAS A&M UNIVERSITY SYSTEM · US
• Gradipore Limited · AU

Inventors

• Gyula Vigh · Magnolia, US
• David Ogle · Redcliffe, AU
• Dennis Rylatt · Melbourne, AU

Key dates

• Filing date: Sep 22, 2001
• Grant date: Aug 2, 2005
• Expiry date: Nov 20, 2021

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N27/44769
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

An electrophoretic apparatus comprising: a first electrolyte chamber containing a first electrode; a second electrolyte chamber containing a second electrode; a first sample chamber disposed between the first and second electrolyte chambers and proximate to the first electrolyte chamber; a second sample chamber disposed between the first sample chamber and the second electrolyte; three ion-permeable barriers separating the first electrolyte chamber, the first sample chamber, the second sample chamber, and the second electrolyte chamber, respectively, wherein the ion-permeable barriers impede convective mixing of the contents in each of the respective chambers; a first electrolyte reservoir and a second electrolyte reservoir in fluid communication with the first and second electrolyte chambers, respectively; a first sample reservoir and a second sample reservoir in fluid communication with the first and second sample chambers, respectively; means adapted for communicating a first electrolyte and a second electrolyte between the respective electrolyte chambers and reservoirs; means adapted for communicating a first fluid and a second fluid between the respective sample chambers and r…