Methods for identifying anti-microbial agents
US6924101B2 · kind B2 · utility
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13References
88Claims
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Key dates
| Filing date | Mar 13, 2001 |
| Grant date | Aug 2, 2005 |
| Priority date | — |
| Expiry date | Jul 20, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/113
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for identifying endogenous microbial proliferation genes for growth and viability is disclosed herein. The method involves exogenous nucleic acids that are used to conditionally produce antisense inhibitors of endogenous complementary mRNAs in a microorganism. Antisense fragments that result in lethality when expressed indicate that the endogenous gene is a proliferation gene. The method can also be used with sequences in sense orientation. The strategy can be used to identify new gene targets for novel antibiotics.
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