Method and kit for isolating DNA probes that bind to activated transcription factors
US6924113B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jun 8, 2001 |
| Grant date | Aug 2, 2005 |
| Priority date | — |
| Expiry date | Jun 8, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6845
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods, arrays and kits are provided for rapidly and efficiently identifying and quantifying multiple different activated transcription factors in a biological sample at the same time. In one aspect, a method is provided for isolating DNA probes which bind to activated transcription factors, including the step of mixing a library of double stranded DNA probes with a sample containing activated transcription factors. The transcription factor probes that have bound to the activated transcription factors are isolated from the mixture via an agarose gel separation. The bound probes can be identified, for example, by using an array of hybridization probes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.