Phosphopeptide-specific antibodies that are activity specific; methods of production and antibody uses
US6924361B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 2, 1993 |
| Grant date | Aug 2, 2005 |
| Priority date | — |
| Expiry date | Nov 2, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K16/32
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to growth regulatory proteins expressed in various disease states, especially receptor tyrosine kinases and similar growth factor receptors. Prior to the Applicants invention, it was not clear that specific antibodies could be generated that recognized peptide epitopes comprising phosphotyrosine in the context of its surrounding amino acid sequence. Applicants generated antibodies to such phosphotyrosine specific peptides, distinct from antibodies that recognize only phosphotyrosine itself. The invention includes methods for producing phosphopeptide specific antibodies by removing contaminating antibody specificities by negative and/or positive selections. Phosphospecific antibodies and their uses in immunodetection, diagnostic or therapeutic applications are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.