Methods and compositions for effecting homologous recombination

US6936418B2 · kind B2 · utility

Assignees

• INSTITUT CURIE · FR
• CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE · FR
• MUSEUM NATIONAL D'HISTOIRE NATURELLE · FR
• Institut National de la Sante et de la Recherche Medicale (INSERM) · FR

Inventors

• Marie Dutreix · L'Haÿ-les-Roses, FR
• Jian Sun · Saint-Maur-des-Fossés, FR
• Elodie Biet · Paris, FR
• Rosalie Maurisse · Paris, FR
• Jean-Paul Feugeas · Brunoy, FR

Key dates

• Filing date: Jan 18, 2002
• Grant date: Aug 30, 2005
• Expiry date: Jan 18, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/902
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention concerns a method for effecting directed mutagenesis based on a guided homologous recombination system that comprises (i) a triple helix forming oligonucleotide, (ii) a donor nucleic acid segment, and (iii) an adapter segment comprising an oligonucleotide sequence able to bind at least a portion of said donor nucleic acid through Watson-Crick base pairing.