Process for the preparation of L-phosphinothricine by enzymatic transamination with aspartate

US6936444B1 · kind B1 · utility

Assignee

• Aventis CropScience GmbH · DE

Inventor

• Klaus Bartsch · Steinbach, DE

Key dates

• Filing date: Jun 30, 2000
• Grant date: Aug 30, 2005
• Expiry date: Jun 30, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P13/04
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The patent application describes a process for the enzymatic chiral synthesis of L-phosphinothricin by transamination from its corresponding keto acid PPO with aspartate as amino donor. It is possible by a suitable reaction procedure to achieve a quantitative conversion on use of approximately equimolar amounts of amino donor and acceptor with complete consumption of the donor amino acid aspartate. The use of thermally stable transaminases makes a higher reaction rate and correspondingly large space/time yields possible.