Method of preparing a biological sample for quantification
US6936473B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 29, 2001 |
| Grant date | Aug 30, 2005 |
| Priority date | — |
| Expiry date | Apr 20, 2022 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25625
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention is directed to a method of preparing a biological sample for quantification which includes an element to be quantified. A volume of biological sample is collected without quantifying the volume thereof to mix with a specified volume of an aqueous solution. Absorptivity of an indicating material in the aqueous solution is measured. Absorptivity of the indicating material in the collected biological sample mixed with the specified volume of the aqueous solution is measured. Next, a dilution ratio of the biological sample is calculated using the absorptivity of the indicating material in the specified volume of the aqueous solution and the absorptivity of the indicating material in the biological sample mixed with the specified volume of the aqueous solution. Absorptivity of the element in the biological sample mixed with the specified volume of aqueous solution is measured and a quantified value of the element in the biological sample is obtained using the measured absorptivity of the element and the calculated dilution ratio.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.