Purification of protein inclusion bodies by crossflow microfiltration
US6936699B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Apr 25, 2001 |
| Grant date | Aug 30, 2005 |
| Priority date | — |
| Expiry date | Apr 25, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/34
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a process for purifying and/or concentrating inclusion bodies (refractile bodies) in a highly concentrated particle-containing solution, in which process the inclusion body-containing suspension is directed tangentially past one or more semipermeable membranes, so that the inclusion bodies are retained behind the membranes and that substances having a relatively small molecular weight may pass through the membrane and/or be adsorbed to the membrane, and a purified and/or concentrated inclusion body suspension is obtained. In the same manner, cell wall particles are removed from a inclusion body suspension. The invention further relates to the use of a crossflow microfiltration unit for purifying and/or concentrating inclusion bodies in a suspension and for removing cell wall particles from a inclusion body preparation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.