Methods for the assay of troponin I and T and complexes of troponin I and T and selection of antibodies for use in immunoassays
US6939678B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 7, 1999 |
| Grant date | Sep 6, 2005 |
| Priority date | — |
| Expiry date | Feb 26, 2021 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/819
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Assay systems and specialized antibodies for the detection and quantitation of troponin I and troponin T in body fluids as an indicator of myocardial infarction. Since troponin I and T exist in various conformations in the blood, the ratios of the monomeric troponin I an T and the binary and ternary complexes, as well as which form of troponin present in the blood, may be related to the metabolic state of the heart. Disclosed is a system to determine the presence of a troponin form or a group of troponin forms in a sample of whole blood, serum or plasma.Disclosed is a stabilized composition of troponin; the stabilized composition can comprise a stabilized composition of troponin I, wherein the troponin I is oxidized, the troponin I can be unbound or the troponin I can be in a complex.Disclosed is a method for improving the recovery of troponin I or T from a surface used in immunoassays.Also disclosed are antibodies which recognize, unbound troponin forms, the forms of troponin in binary complexes, the ternary complex of troponin I, T and C, and the conformations of troponin I having intramolecularly oxidized and reduced cysteines.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.