Micromachined lysing device and method for performing cell lysis
US6942169B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Mar 7, 2002 |
| Grant date | Sep 13, 2005 |
| Priority date | — |
| Expiry date | Mar 30, 2023 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/82
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method and device for performing lysing on a cell-containing fluid, in which the fluid flows through a vibrating micromachined tube to physically rupture the cell walls (mechanical lysis), and/or to mix, agitate or homogenize the fluid during chemical lysis, and/or to mix, agitate or homogenize the lysate for analysis or other processing after lysing. The tube includes a freestanding portion spaced apart from a surface of a substrate on which the tube is formed. The device further includes means for vibrating the freestanding portion of the tube at a level sufficient to rupture the walls of cells in a fluid flowing through the freestanding portion (for mechanical lysing) or to mix the fluid and a chemical lysing additive within the freestanding portion (for chemical lysing).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.