Microstructure apparatus and method for separating differently charged molecules using an applied electric field
US6942778B1 · kind B1 · utility
17Cited by
22References
50Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 28, 2000 |
| Grant date | Sep 13, 2005 |
| Priority date | — |
| Expiry date | Jan 25, 2022 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L3/5027
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The field of the present invention relates generally to a microstructure apparatus which may be used in a high-throughput screening context to monitor the rate of reaction of an enzyme with its substrate in cases where the product of the reaction has an altered net charge. For example, the systems and methods disclosed herein may be used to detect the activity of phosphatase enzymes, proteases and kinases on charged peptide substrates. The microstructure devices of the present invention comprise a plurality of microstructures, wherein each microstructure comprises a capture matrix located between two electrodes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.