Apparatus for analysis of a nucleic acid amplification reaction
US6942971B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 19, 2001 |
| Grant date | Sep 13, 2005 |
| Priority date | — |
| Expiry date | Dec 19, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/0627
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An apparatus for determining a threshold value (e.g., a threshold cycle number or a time value) in a nucleic acid amplification reaction comprises a detection mechanism for measuring, at a plurality of different times during the amplification reaction, at least one signal whose intensity is related to the quantity of a nucleic acid sequence being amplified in the reaction. A controller in communication with the detection mechanism is programmed to store signal values defining a growth curve for the nucleic acid sequence, determine a derivative of the growth curve, and calculate a cycle number or time value associated with a characteristic of the derivative.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.