Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use
US6943279B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 8, 2000 |
| Grant date | Sep 13, 2005 |
| Priority date | — |
| Expiry date | Apr 8, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8282
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera and Rhinocladiella airovirens. The polynucleotides may be mutated to remove glycosylation sites and cysteine residues. Additionally, the present invention provides recombinant expression cassettes, host cells, transgenic plants, and transgenic seed. The present invention also provides for polynucleotides containing both APAO and a fumonisin esterase. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and eukaryotic systems, methods for detecting fumonisins, and methods for identifying transformed plant cells. Methods for degrading fungal toxins in plants, grain, grain processing, silage, food crops and in animal feed are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.