Process for the separation of double-stranded/single-stranded nucleic acid structures

US6946250B2 · kind B2 · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Helge Bastian · Mettmann, DE
• Simone Gauch · Haan, DE
• Metin Colpan · Essen, DE
• Petra Feuser · Cologne, DE

Key dates

• Filing date: Dec 4, 2001
• Grant date: Sep 20, 2005
• Expiry date: Mar 16, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6806
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention pertains to a process for the chromatographic separation of nucleic acid mixtures into their double-stranded and single-stranded nucleic acid fractions by simultaneously absorbing said nucleic acids as a whole to a mineral support, followed by separation into double-stranded and single-stranded nucleic acids by fractional elution, or by selectively absorbing double-stranded or single-stranded nucleic acid of a liquid sample to a mineral support, as well as solutions and a kit for performing the process according to the invention.