Method for monitoring degradation of Hb-based blood substitutes
US6949384B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | May 2, 2002 |
| Grant date | Sep 27, 2005 |
| Priority date | — |
| Expiry date | May 25, 2023 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/72
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a method for monitoring degradation of Hb-based blood substitutes, in a sample. This method involves determining a concentration of met-Hb within the sample, by applying a calibration algorithm for met-Hb to an absorbance obtained from the sample at one or more than one wavelengths, and using the concentration of met-Hb, as a measurement of degradation of the Hb-based blood substitutes. Using this assay, a concentration of met-Hb that is equal to or greater than 3% may be used as an indicator of degradation of Hb. Alternatively, by obtaining samples over a period of time, the concentration of met-Hb and the concentration of Hb-based blood substitute may be determined in each of these samples, and an increase in the concentration of met-Hb over the period of time is an indicator of degradation of Hb. The sample may be a whole blood sample, a serum sample, or a plasma sample obtained from a patient transfused with one or more than one Hb-based blood substitutes, a stock Hb-based blood substitute sample, or a body part of a patient.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.