Primers useful for sizing nucleic acids
US6949633B1 · kind B1 · utility
18Cited by
176References
22Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 25, 1998 |
| Grant date | Sep 27, 2005 |
| Priority date | — |
| Expiry date | Aug 25, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/255
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides modified oligonucleotide primers designed to incorporate a cleavable moiety so that a 3′ portion of the primer (linked to an extension product) can be released from an upstream 5′ portion of the primer. Upon selective cleavage of the cleavable site, primer extension products that contain about five or fewer base pairs of the primer sequence are released, to provide more useful sizing and sequence information per fragment than extension products containing the entire primer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.