Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
US6951719B1 · kind B1 · utility
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36References
23Claims
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Key dates
| Filing date | Nov 28, 2000 |
| Grant date | Oct 4, 2005 |
| Priority date | — |
| Expiry date | Feb 27, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07H21/02
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Ligation-mediated method of recombining polynucleotides in vitro. Polynucleotides from a library are fragmented and the fragments are hybridized to an assembly template. The hybridized fragments are iteratively re-hybridized and ligated until the ends of the hybridized fragments are adjacent to the ends of other hybridized fragments on the assembly template. A final ligation produces recombined polynucleotides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.