Patent · US Expired

Method for amplifying nucleic acid sequence

US6951722B2 · kind B2 · utility

34Cited by
3References
72Claims
0Family size

Assignee

Inventors

Key dates

Filing dateAug 23, 2001
Grant dateOct 4, 2005
Priority date
Expiry dateJun 8, 2022

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6853
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.