High throughput screening method for biological agents affecting fatty acid biosynthesis
US6951729B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 26, 2000 |
| Grant date | Oct 4, 2005 |
| Priority date | — |
| Expiry date | Dec 11, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided is a screening method for compounds affecting fatty acid biosynthesis, the method comprising: providing a reaction mixture comprising: an acyl carrier moiety or enzymes and precursors sufficient to generate the acyl carrier moiety; a bacterial enzymatic pathway comprising at least two consecutively acting enzymes selected from the group consisting of: malonyl-CoA:ACP transacylase, beta-ketoacyl-ACP synthase III, NADPH dependent beta-ketoacyl-ACP reductase, beta-hydroxylacyl-ACP dehydrase and enoyl-ACP reductase; and substrates and cofactors required for the operation of the enzymes; contacting the reaction mixture with a prospective bioactive agent; conducting a high throughput measurement of the activity of the enzymatic pathway; and determining if the contacting altered the activity of the enzymatic pathway.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.