DNA and amino acid sequences of a tyrosine-inducible tyrosine ammonia lyase enzyme from the yeast Trichosporon cutaneum

US6951751B2 · kind B2 · utility

Assignee

• E.I. DU PONT DE NEMOURS AND COMPANY · US

Inventors

• Sabine Breinig · Philadelphia, US
• Wei Qi · Beijing, CN
• Fateme Sima Sariaslani · Wilmington, US
• Todd Vannelli · Ithaca, US
• Zhixiong Xue · Chadds Ford, US

Key dates

• Filing date: May 16, 2003
• Grant date: Oct 4, 2005
• Expiry date: May 28, 2023

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/88
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A novel tyrosine-inducible tyrosine ammonia lyase enzyme was isolated from the yeast Trichosporon cutaneum. This enzyme has a higher activity for tyrosine than for phenylalanine and is useful for the production of para-hydroxycinnamic acid directly from tyrosine. The gene encoding this enzyme was sequenced using 3′ and 5′ RACE cloning of the TAL cDNA and the gene was expressed in the yeast Saccharomyces cerevisiae and in the bacterium Escherichia coli.