Dye compositions which provide enhanced differential fluorescence and light scatter characteristics
US6955872B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 20, 2003 |
| Grant date | Oct 18, 2005 |
| Priority date | — |
| Expiry date | Oct 12, 2023 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2015/1477
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A composition for enhancing differential staining of RNA, DNA and granules in a sample comprising cells contains a first fluorescent dye that can bind specific binding sites and non-specific binding sites in the sample. This first dye emits fluorescence at a first wavelength. The composition contains at least an additional component, which is a second non-intercalating dye in the composition that competes with said first dye for binding to the nonspecific binding sites, or a permeabilizing agent to enhance permabilization of the dyes into the cells, or both. The molar ratio of the second dye and the first dye is at least about 20:1.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.