Compositions and systems for identifying and comparing expressed genes (mRNAs) in eukaryotic organisms
US6955876B2 · kind B2 · utility
2Cited by
3References
32Claims
0Family size
Inventors
Key dates
| Filing date | Nov 1, 2001 |
| Grant date | Oct 18, 2005 |
| Priority date | — |
| Expiry date | Nov 24, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6809
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention comprises compositions and systems to identify and compare expressed genes in a given in vivo or in vitro RNA sample, as well as the relative difference in mRNA expression between two or more samples, where desired. Furthermore, the invention comprises compositions and systems to identify novel genes. The invention comprises, without limitation, one or more mRNA specific identimers for use in reverse transcription that themselves comprise an oligo-T nucleotide sequence (at the 5′ end) linked to a nucleotide sequence VNx (at the 3′ end) where the V nucleotide immediately adjacent to the oligo-T segment is not a T.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.