System and method for increasing the contrast of an image produced by an epifluorescence microscope
US6956695B2 · kind B2 · utility
5Cited by
52References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 19, 2002 |
| Grant date | Oct 18, 2005 |
| Priority date | — |
| Expiry date | Mar 19, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B21/34
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The contrast of an image produced by epifluorescence microscopy may be increased by placing a high-pass dichroic reflecting film behind the sample. The reflecting film reflects the emission light emitted by the fluorescent tags in the sample back through the objective lens while allowing the shorter wavelength excitation light to pass through the sample holder.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.