Three component chimeric antisense oligonucleotides
US6958239B2 · kind B2 · utility
7Cited by
5References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 15, 1998 |
| Grant date | Oct 25, 2005 |
| Priority date | — |
| Expiry date | Dec 15, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/346
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to antisense oligonucleotides that target mRNAs in cells as substrates for the cellular enzyme RNase H and thereby cause specific degradation of the targeted mRNA. The oligonucleotides have three components: an RNase H activating region, a complementary region and 3′ and 5′ ends. The invention optimizes each of the components to resist intracellular nucleases, to increase hybridization to target mRNA, to specifically inactivate target mRNA in cells, and to decrease cytotoxicity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.