Method for the detection of dyes in fluorescence microscopy

US6958811B2 · kind B2 · utility

Assignee

• Carl Zeiss Jena GmbH · DE

Inventors

• Ralf Wolleschensky · Jena, DE
• Gunter Moehler · Jena, DE

Key dates

• Filing date: Jun 28, 2001
• Grant date: Oct 25, 2005
• Expiry date: Jun 28, 2021

Classification

• Technology area (CPC G): Physics
• CPC primary: G02B21/16
• WIPO field: Optics
• WIPO sector: Instruments

Abstract

A method for optical detection of characteristic quantities of the wavelength-dependent behavior of an illuminated specimen, such as the emission behavior and/or absorption behavior, preferably the fluorescence and/or enzyme fluorescence comprises determining at least one spectral centroid and/or a maximum of the emission radiation and/or of the absorbed radiation. An arrangement for performing the method is disclosed.