Patent · US Expired

Methods for lyophilizing competent cells

US6960464B2 · kind B2 · utility

62Cited by
25References
13Claims
0Family size

Assignee

Inventors

Key dates

Filing dateApr 11, 2002
Grant dateNov 1, 2005
Priority date
Expiry dateApr 11, 2022

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S435/849
  • WIPO fieldBasic materials chemistry
  • WIPO sectorChemistry

Abstract

This invention relates to a method for producing cells which are competent for transformation and which may be stably stored for extended periods of time at various temperatures. The method involves growing cells in a growth conducive medium, rendering said cells competent, and lyophilizing said competent cells. The invention further relates to competent cells produced by such a method, to methods of transforming said cells with a DNA molecule, and to a method of producing a desired protein or polypeptide from said transformed cells.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.