Patent · US Expired

Method for synthesis of nucleic acids

US6962780B2 · kind B2 · utility

7Cited by
11References
20Claims
0Family size

Assignee

Inventors

Key dates

Filing dateApr 30, 2001
Grant dateNov 8, 2005
Priority date—
Expiry dateAug 8, 2021

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/686
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present invention is a method for synthesis of nucleic acids to amplify an intended nucleic acid in a region in which a GC content is rich, wherein a polyhydric alcohol and/or ammonium sulfate is present in an amplification reaction solution. According to the present invention, it is possible to amplify nucleic acids in a GC rich region efficiently and directly from a sample such as blood containing lots of PCR inhibitory substances without undergoing a process of isolating and purifying the nucleic acid, even though conducting PCR in the GC rich region tends to be difficult using conventional processes even if purified DNA is used.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.