Process for production diphtheria toxin

US6962803B2 · kind B2 · utility

Assignee

• Amersham Health AS · NO

Inventors

• Henry Wolfe · Lower Burrell, US
• Fahar Merchant · Vancouver, CA
• Rosemina Merchant · Edmonton, CA
• Christopher Black · Norristown, US
• Harry Storflor · Oslo, NO
• Geir Stokke · Oslo, NO
• Halldis Hellebust · Oslo, NO

Key dates

• Filing date: Oct 22, 2003
• Grant date: Nov 8, 2005
• Expiry date: Oct 22, 2023

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/55
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention provides a method of purifying diphtheria toxin comprising (1) fermenting a microorganism strain capable of producing diphtheria toxin using glucose as a carbon source, said method comprising adding glucose to a growing culture whereby the addition of glucose maintains microorganism growth effective to support diphtheria toxin production; and (2) purifying the diphtheria toxin from the culture by contacting a toxin containing preparation derived therefrom with an ion exchange matrix, eluting a fraction containing the toxin, applying the eluate to a hydrophobic matrix, and eluting a fraction containing the toxin.