Methods for producing high titre vectors and compositions used in such methods
US6969598B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Apr 30, 2002 |
| Grant date | Nov 29, 2005 |
| Priority date | — |
| Expiry date | Apr 17, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/48
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for producing viral vectors is described using packaging and producer cell lines is described. The producer cell comprises: (i) a first nucleotide sequence (NS) encoding a toxic viral envelope protein operably linked to a promoter; wherein the promoter is operably linked to at least one copy of a TRE; (ii) a second NS wherein the second NS comprises a sequence encoding a tetracycline modulator; (iii) a third NS encoding a retrovirus nucleocapsid protein; and (iv) a fourth NS comprising a retroviral sequence capable of being encapsidated in the nucleocapsid protein such that the retroviral vector particle titre obtainable from the producer cell is regulatable by tetracycline and an initial stimulus with sodium butyrate or functional analogues thereof.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.