Capillary array-based enzyme screening
US6972183B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 22, 1999 |
| Grant date | Dec 6, 2005 |
| Priority date | — |
| Expiry date | Nov 22, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1086
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for screening an expression library to identify clones expressing enzymes having a desired activity is provided. The process involves first generating from genomic DNA samples of one or more microorganisms an expression library comprising a plurality of recombinant cell clones, and then introducing into capillaries in a capillary array a substrate and at least a subset of the clones, either individually or as a mixture. Interaction of the substrate and a clone expressing an enzyme having the desired activity produces an optically detectable signal, which can then be spatially detected to identify capillaries containing clones producing such a signal. The signal-producing clones can then be recovered from the identified capillaries.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.