Patent · US Expired

Skin substitutes with improved barrier function

US6974697B2 · kind B2 · utility

26Cited by
9References
4Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMar 1, 2002
Grant dateDec 13, 2005
Priority date
Expiry dateMar 31, 2022

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2533/54
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present invention relates to in vitro cultured skin substitutes, and in particular to in vitro cultured skin substitutes that have improved barrier function. In some embodiments, improved barrier function is a result of improved culture conditions, while in other embodiments, improved barrier function results from genetic modification of keratinocytes. Improved culture conditions to improve barrier function include organotypic culture in the presence of linoleic acid and/or linoleic acid at about 75% humidity. Suitable genetic modifications for improving barrier function includes transfection with a DNA construct capable of expressing GKLF.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.