Biological assays using coded RNA reporters
US6977152B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 9, 2002 |
| Grant date | Dec 20, 2005 |
| Priority date | — |
| Expiry date | Apr 15, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6897
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Systems for multiplexed analysis of gene expression using coded RNA reporters. The coded RNA reporters are expressed from a library of reporter genes, with each reporter gene directing expression of a distinguishable coded RNA reporter. Each RNA reporter thus reports activity of a corresponding reporter gene. The distinguishable reporter RNA species allow otherwise similar reporter genes to be functionally linked to different effectors within a cell reporter library. Multiplexed analysis of reporter gene activity is carried out by measuring the level of each RNA reporter within the cell reporter library, thus providing an ability to analyze the effect of a biological modulator on many effectors.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.