Solid support assay systems and methods utilizing non-standard bases

US6977161B2 · kind B2 · utility

Assignee

• Eragen Biosciences, Inc. · US

Inventors

• Jennifer K. Grenier · Madison, US
• David J. Marshall · Elmore, AU
• James R. Prudent · Madison, US
• Craig S. Richmond · Madison, US
• Eric B. Roesch · Oregon, US
• Christopher W. Scherrer · Rio, US
• Christopher B. Sherrill · Madison, US
• Jerod PTACIN · Beloit, US

Key dates

• Filing date: Oct 15, 2001
• Grant date: Dec 20, 2005
• Expiry date: Oct 15, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target oligonucleotide. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorporation of a non-standard base (e.g., via PCR or ligation) is used in the assay.