Electrophoretic method and system having internal lane standards for color calibration
US6982029B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 7, 2001 |
| Grant date | Jan 3, 2006 |
| Priority date | — |
| Expiry date | Aug 25, 2023 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44726
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An electrophoretic system having a plurality of separation lanes is provided with an automatic color calibration feature in which each lane is separately calibrated. For each lane, a dye matrix standard is created using reference fragments which migrate either before, or after, the sample fragments being electrophoresced, during the same electrophoresis run. These dye standards are detected automatically for the purpose of determining coefficients used to identify sample fragments mixed together with the reference fragments. This allows for calibrating the dye standard matrix for each of a plurality of lanes each time the electrophoresis system is used.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.