HEK293 cell line for activity studies and HTS system of α1G T-type Ca2+ channel
US6984521B2 · kind B2 · utility
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Key dates
| Filing date | May 28, 2004 |
| Grant date | Jan 10, 2006 |
| Priority date | — |
| Expiry date | May 28, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2503/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
There is provided a cell line that is prepared by transforming HEK293 cell with a human Kir2.1 gene using a retrovirus expression system, wherein the HEK293 expresses a stable α1G T-type calcium channel. The cell line responds sensitively to KCl and forms an appropriate level of the membrane voltage so that the cell signaling pathway may be investigated by the molecular biological and biochemical studies.
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