Patent · US Expired

Method for enrichment of natural antisense messenger RNA

US6986988B2 · kind B2 · utility

109Cited by

1References

18Claims

0Family size

Assignee

Quark Biotech, Inc. · US

Inventors

Shlomit Gilad · Ganei Hadar, IL
Paz Einat · Nes Ziona, IL
Avital Grossman · Nes Ziona, IL

Key dates

Filing date	Apr 11, 2001
Grant date	Jan 17, 2006
Priority date	—
Expiry date	Jun 28, 2023

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2330/10
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method for enrichment of natural antisense mRNA which involves hybridization of cDNA obtained from sense RNA with cDNA obtained from antisense RNA, followed by DNA polymerase treatment of the sense-antisense hybrid DNA molecule. A natural antisense library can be generated by cloning of sense-antisense hybrid DNA molecules in a vector.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.