High-throughput screen for identifying channel blockers that selectively distinguish transient from persistent sodium channels
US6991910B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 20, 2001 |
| Grant date | Jan 31, 2006 |
| Priority date | — |
| Expiry date | Dec 31, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/10
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for identifying a Na+ channel blocker, including providing a cell containing a Na+ channel, demonstrating both a transient and a persistent current. The cell includes a potassium (K+) channel and a Na/K ATPase (Na+ pump). A fluorescent dye is disposed into the well. The fluorescent dye is sensitive to change in cell membrane potential in order to enable optical measurement of cell membrane potential. A Na+ channel blocker, to be identified, is added to the well and a stimulating current is passed through the cell in an amount sufficient to generate an action potential before and after the addition of the Na+ channel blocker. Thereafter, a change in cell membrane potential is optically measured.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.