Method comprising the indirect electrochemical regeneration of NAD(P)H

US6991926B2 · kind B2 · utility

Assignee

• BASF SE

Inventors

• Eberhard Steckhan · Meckenheim, DE
• Frank Hollmann · Velen, DE
• Bernhard Hauer · Neheim, DE
• Thomas Zelinski · Menasha, US
• Andreas Schmid · Berkeley, US

Key dates

• Filing date: May 16, 2001
• Grant date: Jan 31, 2006
• Expiry date: Jun 27, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P1/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method comprising the indirect electrochemical regeneration of NAD(P)H in enzymatic substrate conversions which are catalyzed by monooxygenases, for example, is described. In particular, a method for the electroenzymatic preparation of 2,3-dihydroxyphenyl derivatives, which is catalyzed by the enzyme 2-hydroxybiphenyl 3-monooxygenase and in which simultaneously the NAD+ formed by reductive cleavage of oxygen is electrochemically reduced, is described.