Diagnostic sequencing by a combination of specific cleavage and mass spectrometry
US6994969B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 30, 2000 |
| Grant date | Feb 7, 2006 |
| Priority date | — |
| Expiry date | Apr 30, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6855
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is in the field of nucleic acid-based diagnostic assays. More particularly, it relates to methods useful for the “diagnostic sequencing” of regions of sample nucleic acids for which a prototypic or reference sequence is already available (also referred to as “re-sequencing”), or which may be determined using the methods described herein. This diagnostic technology is useful in areas that require such re-sequencing in a rapid and reliable way: (i) the identification of the various allelic sequences of a certain region/gene, (ii) the scoring of disease-associated mutations, (iii) the detection of somatic variations, (iv) studies in the field of molecular evolution, (v) the determination of the nucleic acid sequences of prokaryotic and eukaryotic genomes, (vi) identifying one or more nucleic acids in one or more biological samples', (vii) and determining the expression profile of genes in a biological sample and other areas.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.