Packaging cell
US6995009B1 · kind B1 · utility
18Cited by
0References
17Claims
0Family size
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Inventors
Key dates
| Filing date | Jun 1, 2000 |
| Grant date | Feb 7, 2006 |
| Priority date | — |
| Expiry date | Oct 28, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/10052
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A virus-producing cell sustaining the ability to produce viruses at high titer is successfully constructed by expressing the virus structural gene under the regulation of EF1α promoter. In this virus-producing cell, the virus structural gene is ligated to a selection marker gene via IRES and domains other than the protein coding domain are eliminated from the DNA encoding virus structural proteins. Thus, reduction of the titer due to cell passages can be prevented and emergence of wild type viruses caused by unfavorable recombination of the virus genome can be inhibited.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.