Process for inducing direct somatic embryogenesis in immature scutella cells of pooideae, and rapidly regenerating fertile plants
US6995016B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 14, 2001 |
| Grant date | Feb 7, 2006 |
| Priority date | — |
| Expiry date | Jun 12, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8279
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process is provided for inducing direct somatic embryogenesis in Pooideae and rapidly regenerating fertile plants by first culturing isolated immature scutella cells in culture medium comprising auxin, cytokinin and polyamine in amounts effective to cause direct formation of primary embryos without an intervening callus stage, at least until at least one primary embryo reaches the globular developmental stage, the auxin being present in greater proportion than cytokinin. A second step includes either a) culturing the primary embryos under conditions to regenerate plantlets, and culturing the primary embryos in regeneration medium; or b) culturing the primary embryos at the globular developmental stage and no longer than the coleoptilar stage in culture medium comprising auxin, cytokinin, and polyamine in amounts effective to cause induction of secondary embryo formation, at least until secondary embryogenesis is detected, the cytokinin being present in greater proportion than auxin, and culturing the secondary embryos under conditions to regenerate plantlets.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.