Propagation of human hepatocytes in non-human animals
US6995299B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 15, 2001 |
| Grant date | Feb 7, 2006 |
| Priority date | — |
| Expiry date | Mar 24, 2023 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/18
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention relates to the preparation of non-human animals having chimeric livers, whereby some or substantially all of the hepatocytes present are human hepatocytes. It is based, at least in part, on the discovery that rats, tolerized in utero against human hepatocytes, were found to serve as long-term hosts for human hepatocytes introduced post-natally, and the introduced hepatocytes maintained their differentiated phenotype, as evidenced by continued production of human albumin. The present invention further relates to the use of such animals as models of various liver diseases, including viral invention. Such embodiments are based on the discovery that transplanted human hepatocytes in chimeric livers were found to be susceptible to Hepatitis B virus and Hepatitis C virus infection.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.