Recombinant proteolytic tryptases, active site mutants thereof, and methods of making same
US6998260B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 21, 2000 |
| Grant date | Feb 14, 2006 |
| Priority date | — |
| Expiry date | May 29, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/815
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method of expressing enzymatically-active, recombinant proteolytic tryptase in a eukaryotic host cell, expression constructs which drive the production of enzymatically-active tryptase in transformed hosts, and genetically-engineered eukaryotic host cells containing the expression constructs and which express enzymatically-active proteolytic tryptases. Uses for the proteolytic tryptases so produced are also disclosed. Also disclosed is a method of making active site mutants of proteolytic tryptases in a eukaryotic host cell, expression constructs which drive the production of the mutants in transformed eukaryotic host cells, and genetically-engineered eukaryotic host cells containing the expression constructs and which express the active-site mutated form of proteolytic tryptases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.